[caret-users] ROIs from freesurfer?

j janssen joost.janssen76 at gmail.com
Wed Sep 17 12:11:41 CDT 2008


Hi,

thanks Donna for your great reply to my previous mail. 2 additional
questions: if i had a (binary) mask of adjacent cortical (grey matter)
segmentations from freesurfer (for example, superior and middle temporal
gyrus).

could i use this mask as a ROI in caret? if so, how would i go about
creating a Caret-readable-usable ROI from this binary nifti mask?

thus, i would import pial, white, and inflated surfaces from FSS into caret,
do the necessary steps to make the surface-shape file and then
-surface-roi-statistical-report using the ROI mask.

does this make sense?

thanks,
-joost


Hi Joost,

The Fornito paper used a ROI approach, i.e., the cingulate sulcus. It looks
like they took great pains to define the region consistently across
subjects, as is the norm; ROI definition (and consistent delineation) is
often the hard part. Assuming Freesurfer 4.05 is new enough to have the
cortical parcellation, perhaps you can use it to define your cingulate
sulcus.

In caret-land, you can then approach things two ways:

* Without registration: Skip registration. Somehow export a parcellation
from Freesurfer that, for each node, assigns 0 to nodes outside the ROI, 1
to nodes inside it. Then, use caret_command -surface-roi-statistical-report
and each subject's surface_shape file to output the mean depth in the ROI. *
With registration: Register your subjects to the PALS atlas. Somehow represent
the ROI in the PALS standard mesh (perhaps by creating a probabilistic atlas
of registered Freesurfer parcellations and then thresholding it). Then, use
caret_command -surface-roi-statistical-report and each subject's
surface_shape file to output the mean depth in the ROI. The advantage of
this route is that you can do more than just output a summary stat for an
entire region, which may not be very informative. You can instead create a
t-map (or f-map) across groups/hemispheres, which gives you greater
spatial specificity
(e.g., see tmap figure in http://dx.doi.org/10.1016/j.neuroimage.2008.07.034).
The latter can be done on either an entire hemisphere or restricted to a ROI
(the latter being represented by a custom CUT topo file that excludes nodes
outside the region). While registration gives you more analytic options, it
also is work. We have an automated border drawing script, but it's not ready
for public consumption yet. For now, you still need to register as defined
here:

http://brainvis.wustl.edu/help/landmarks_core6/landmarks_core6.html/
http://brainmap.wustl.edu/caret/pdf/Caret_5.5_Tutorial_Segment.pdf

Attached is a list of potentially useful caret_command features.

Donna

On 09/05/2008 08:35 AM, j janssen wrote:

Hi,

i´m using Caret5.6.

i´ve used Freesurfer (4.05) to create white and pial surfaces.

my goal is to use these surfaces to get measures of sulcal depth for a brain
region (either a hemisphere or a specific sulcus), so that these can be
statistically compared, such as in Fornito et al 2008, HBM 29: 478-489.
after various steps i´ve succesfully used "caret_command
-surface-identify-sulci"
on the brains in my dataset. does anybody have some pointers as how to
proceed from this point?

thanks a lot,
-joost

------------------------------------------------------------------------

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      caret_command -extend
      caret_command -file-convert
      caret_command -file-substitution
      caret_command -image-combine
      caret_command -image-compare
      caret_command -image-format-convert
      caret_command -image-insert-text
      caret_command -image-resize
      caret_command -image-to-web-page
      caret_command -image-view
      caret_command -metric-clustering
      caret_command -metric-composite
      caret_command -metric-composite-identified-columns
      caret_command -metric-correlation-coefficient-map
      caret_command -metric-file-create
      caret_command -metric-in-group-difference
      caret_command -metric-information
      caret_command -metric-math-postfix
      caret_command -metric-multiple-correlation-coefficient-map
      caret_command -metric-set-column-name
      caret_command -metric-set-column-to-scalar
      caret_command -metric-smoothing
      caret_command -metric-statistics-anova-one-way
      caret_command -metric-statistics-anova-two-way
      caret_command -metric-statistics-coordinate-difference
      caret_command -metric-statistics-interhemispheric-clusters
      caret_command -metric-statistics-kruskal-wallis
      caret_command -metric-statistics-levene-map
      caret_command -metric-statistics-normalization
      caret_command -metric-statistics-one-sample-t-test
      caret_command -metric-statistics-paired-t-test
      caret_command -metric-statistics-shuffled-cross-correlation-map
      caret_command -metric-statistics-shuffled-t-map
      caret_command -metric-statistics-subtract-group-average
      caret_command -metric-statistics-t-map
      caret_command -metric-statistics-two-sample-t-test
      caret_command -metric-statistics-z-map
      caret_command -metric-twin-comparison
      caret_command -metric-twin-paired-data-diffs
      caret_command -paint-add-columns
      caret_command -paint-assign-nodes-relative-to-line
      caret_command -paint-assign-to-nodes
      caret_command -paint-composite
      caret_command -paint-file-create
      caret_command -scene-create
      caret_command -script-convert
      caret_command -script-run
      caret_command -script-variable-read
      caret_command -script-variable-set
      caret_command -show-scene
      caret_command -show-surface
      caret_command -show-volume
      caret_command -spec-file-add
      caret_command -spec-file-clean
      caret_command -spec-file-copy
      caret_command -spec-file-create
      caret_command -spec-file-directory-clean
      caret_command -spec-file-zip
      caret_command -statistic-set-random-seed
      caret_command -statistic-unit-test
      caret_command -stereotaxic-spaces
      caret_command -study-metadata-duplicates
      caret_command -surface-affine-regression
      caret_command -surface-align-to-standard-orientation
      caret_command -surface-apply-transformation-matrix
      caret_command -surface-average
      caret_command -surface-border-create-parallel-border
      caret_command -surface-border-cutter
      caret_command -surface-border-delete
      caret_command -surface-border-draw-around-roi
      caret_command -surface-border-draw-geodesic
      caret_command -surface-border-draw-metric
      caret_command -surface-border-intersection
      caret_command -surface-border-landmark-identification
      caret_command -surface-border-link-to-focus
      caret_command -surface-border-merge
      caret_command -surface-border-nibbler
      caret_command -surface-border-projection
      caret_command -surface-border-resample
      caret_command -surface-border-reverse
      caret_command -surface-border-unprojection
      caret_command -surface-border-variability
      caret_command -surface-crossover-check
      caret_command -surface-curvature
      caret_command -surface-foci-create
      caret_command -surface-foci-delete
      caret_command -surface-foci-projection
      caret_command -surface-foci-unprojection
      caret_command -surface-folding-measures
      caret_command -surface-generate-inflated
      caret_command -surface-identify-sulci
      caret_command -surface-inflate
      caret_command -surface-information
      caret_command -surface-place-foci-at-limits
      caret_command -surface-place-focus-at-extremum
      caret_command -surface-region-of-interest-selection
      caret_command -surface-roi-coord-report
      caret_command -surface-roi-statistical-report
      caret_command -surface-smoothing
      caret_command -surface-sulcal-depth
      caret_command -surface-sulcal-identification-probabilistic
      caret_command -surface-to-cerebral-hull
      caret_command -surface-to-segmentation-volume
      caret_command -surface-to-volume
      caret_command -surface-topology-disconnect-nodes
      caret_command -surfaces-to-segmentation-volume-mask

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On Fri, Sep 5, 2008 at 3:35 PM, j janssen <joost.janssen76 at gmail.com> wrote:

> Hi,
>
> i´m using Caret5.6.
>
> i´ve used Freesurfer (4.05) to create white and pial surfaces.
> my goal is to use these surfaces to get measures of sulcal depth for a
> brain region (either a hemisphere or a specific sulcus), so that these can
> be statistically compared, such as in Fornito et al 2008, HBM 29: 478-489.
> after various steps i´ve succesfully used "caret_command
> -surface-identify-sulci" on the brains in my dataset. does anybody have some
> pointers as how to proceed from this point?
>
> thanks a lot,
> -joost
>
>
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